Journal: Journal of Traditional and Complementary Medicine
Article Title: Xinbao pill attenuated water retention by regulating the CaSR/AQP2 pathway in LAD-induced chronic heart failure rats
doi: 10.1016/j.jtcme.2024.07.002
Figure Lengend Snippet: XBP suppressed renal AQP2 expression via promoting CaSR/p38-MAPK-mediated AQP2 ubiquitination and regulating CaSR/CREB-mediated AQP2 translation. (A and B) The protein expression of p-p38MAPK/p38MAPK in renal tissue from CHF rats (n = 6). (C and D) The protein expression of p-S261 AQP2 in renal tissue from CHF rats (n = 6). (E and F) The ubiquitination of AQP2 in renal tissue from CHF rats (n = 3). (G and H) The protein expression of p-CREB/CREB in renal tissue from CHF rats (n = 6). One-way nested ANOVA followed Dunnett's T3 test was used for multiple groups comparisons (B), and One-way nested ANOVA followed LSD test was used for multiple groups comparisons (D, F, H), the data are presented as Mean ± SEM, sham vs. model group, # p < 0.05, ## p < 0.01, ### p < 0.001; XBP(L/M/H) or QLQX vs. model group, * p < 0.05, ** p < 0.01, *** p < 0.001.
Article Snippet: Antibody against p38MAPK (#14064-1-AP) were bought from Proteintech Group, Inc (Chicago, USA).
Techniques: Expressing, Ubiquitin Proteomics